Aspée Lamas Alexis

Email: 
alexis.aspee@usach.cl
Teléfono: 
27 181 133

Abstract

The extent of the oxidative damage induced by reactive oxygen species (ROS) in biological systems depends on a variety of factors that include the location of the target molecule with respect to the site of ROS formation, the reaction's rate constant, and the presence of antioxidants capable of ROS scavenging and/or repairing the initial damaged molecule. The complexity of these processes requires the development of new tools and strategies to pinpoint the free radical processes affecting a biological system. During the last few years our group has focused its research on developing new strategies to study free radical processes and mechanisms involved in amino acid and protein oxidation, such as spontaneous weak level chemiluminescence. Recently, we developed and applied nitroxide pre-fluorescent probes to monitor free radical formation in a wide variety of chemical systems, including zeolites, polymer films, and proteins. Furthermore, the use of these probes was extended to evaluate polyphenolic antioxidants in hydrogen and/or electron transfer-based reactions towards nitroxides, both in homogeneous and in microheterogeneous systems. These methodologies have allowed us to understand better antioxidant and free radical oxidation mechanisms in complex systems in concomitance with the use of time resolve techniques to monitor transient and free radical formation photoinduced by laser excitation of molecular probes and their reaction with proteins, amino acids, and also nitroxide and phenolic antioxidants.

Jerarquía: 
Asociado
Línea de investigación: 
Design and use of Nitroxide prefluorescent probes to study free radical and antioxidants in biological systems. Mechanisms of protein and amino acid oxidation mediated by reactive oxygen species. Photoinduced free radical formation and their interaction w
Grados académicos /educación: 
Químico, Universidad de Santiago de Chile, 1994
Doctor en Química, Universidad de Santiago de Chile, 2000 Postdoctorado: University of Ottawa, Canadá, 2001-2003